Induced Breeding and Embryonic Development of Climbing Perch (Anabas testudineus, Bloch)
This study described the induced breeding and embryonic stage of Anabas testudineus using commercial hormone LHRHa with the intensity level of 2, 20, 200 μg kg-1 of body weight, respectively. It was found that all intensity of LHRHa hormone level could enhance the fish to breed with the exception of the control group. Fertilization rate, hatching rate, latency period, fecundity, oocytes diameter and GSI were quantified in each set of experiment. It was observed that the fertilised eggs of A. testudineus were almost spherical in shape, clear pearl likes in appearance and free floating on water surface. Fecundity and GSI were significantly higher in fish treated with 200 μg kg-1 as compared to fish treated with 2 and 20 μg kg-1 of body weight of LHRHa hormone. There was no significant (p>0.05) effect between hormone level on fertilization rate, hatching rate and eggs diameter. The diameters of fertilised eggs ranged from 800-850 μm. The first cleavage occurred at 1:30 h, epiboly began at 5 h, the embryonic body was formed at 12 h and hatching occurred at 20 h after fertilization at water temperature of 26°C. Newly hatched larvae were approximately 0.6-1 mm (total length) and pigment spots were present over the yolk and head. The embryonic development of the fish is described. The present study indicated that the administration of LHRHa hormone is effective for ovulation and hormone level at 2 μg kg-1 is recommended.
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