Asian Science Citation Index is committed to provide an authoritative, trusted and significant information by the coverage of the most important and influential journals to meet the needs of the global scientific community.  
ASCI Database
308-Lasani Town,
Sargodha Road,
Faisalabad, Pakistan
Fax: +92-41-8815544
Contact Via Web
Suggest a Journal
The Journal of Experimental Medicine
Year: 2010  |  Volume: 207  |  Issue: 12  |  Page No.: 2703 - 2717

Mouse CD8{alpha}+ DCs and human BDCA3+ DCs are major producers of IFN-{lambda} in response to poly IC

H Lauterbach, B Bathke, S Gilles, C Traidl Hoffmann, C. A Luber, G Fejer, M. A Freudenberg, G. M Davey, D Vremec, A Kallies, L Wu, K Shortman, P Chaplin, M Suter, M O'Keeffe and H. Hochrein    

Abstract:

Polyinosinic:polycytidylic acid (poly IC), a double-stranded RNA, is an effective adjuvant in vivo. IFN-s (also termed IL-28/29) are potent immunomodulatory and antiviral cytokines. We demonstrate that poly IC injection in vivo induces large amounts of IFN-, which depended on hematopoietic cells and the presence of TLR3 (Toll-like receptor 3), IRF3 (IFN regulatory factor 3), IRF7, IFN-I receptor, Fms-related tyrosine kinase 3 ligand (FL), and IRF8 but not on MyD88 (myeloid differentiation factor 88), Rig-like helicases, or lymphocytes. Upon poly IC injection in vivo, the IFN- production by splenocytes segregated with cells phenotypically resembling CD8+ conventional dendritic cells (DCs [cDCs]). In vitro experiments revealed that CD8+ cDCs were the major producers of IFN- in response to poly IC, whereas both CD8+ cDCs and plasmacytoid DCs produced large amounts of IFN- in response to HSV-1 or parapoxvirus. The nature of the stimulus and the cytokine milieu determined whether CD8+ cDCs produced IFN- or IL-12p70. Human DCs expressing BDCA3 (CD141), which is considered to be the human counterpart of murine CD8+ DCs, also produced large amounts of IFN- upon poly IC stimulation. Thus, IFN- production in response to poly IC is a novel function of mouse CD8+ cDCs and their human equivalents.

View Fulltext    |   Related Articles   |   Back
   
 
 
 
  Related Articles

No Article Found
 
 
 
Copyright   |   Desclaimer   |    Privacy Policy   |   Browsers   |   Accessibility