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Journal of Entomology
Year: 2020  |  Volume: 17  |  Issue: 2  |  Page No.: 48 - 57

Biological Control of Aonidiella aurantii (Hemiptera-Diaspididae) by the Entomopathogenic Fungi Aspergilllus flavus and Fusarium oxysporum

Lamharchi El Hassane, Zahidi AbdelAziz, Hallouti Ayoub, Akchour Abdellah, El Finti aissam, Boubaker Hassan and El Mousadik Abdelhamid    

Abstract: Background and Objective: The California Red Scale (CRS) is a serious pest of citrus in many parts of the world. Different control methods have been adopted, but their effectiveness remains limited. The objective was to isolate fungi from the CRS corpses and determine the efficacy of two isolated strains, Aspergilllus flavus and Fusarium oxysporum against different CRS developmental stages. Materials and Methods: Fungal isolates obtained from RSC corpses were identified by studying their macroscopic and microscopic aspects. Mycelial biomass was used for genomic DNA extraction according to kit protocol. Polymerase Chain Reaction (PCR) with specific primers on a partial sequence was used for each fungal isolate. Each PCR product was sequenced in the forward and reverse directions using an Applied Biosystem ABI 3130×l sequencer. Results: The result of the study informs that most isolated strains belonged to five genera of Aspergillus, Alternaria, Fusarium, Rhizopus and Cladosporium. At concentration of 106 conidia mL1, pre-nymph male and adult male under shield showed greater mortality ranging from 84.02-93%, but for female stages, this rate did not exceed 58%. Adult males, male pre-pupae and mobile larvae (68.3-100%) are more affected by Fusarium than Aspergillus strain. Conclusion: It can be concluded that the two entomopathogenic fungi tested can be considered as good candidates for biological control of California Red Scale (CRS). Therefore, they will be used as microbial agents in Integrated Pest Management (IPM) strategies.

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