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Journal of Entomology
Year: 2015  |  Volume: 12  |  Issue: 2  |  Page No.: 77 - 86

Biochemical and Physiological Effects of Lufenuron and Chlorfluazuron on Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae)

Hamdy K. Abou- Taleb, Hossam El-Din M. Zahran and Abir A. Gad    

Abstract: Toxicity and effects of lufenuron and chlorfluazuron on some biochemical and physiological parameters of Spodoptera littoralis (Boisd.) larvae were assessed. Results showed no significant differences in toxicity of lufenuron and chlorfluazuron against 4th instar larvae with LC50 values 1.7 and 2.2 ppm, respectively. While, activity level of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP) increased, activity level of glutathione S-transferase (GST) showed adverse results after exposure to lufenuron and chlorfluazuron compared with control. Lufenuron at 0.62 ppm and chlorfluazuron at 0.76 ppm caused the highest increase in the AST activity (from 1.06 in control to 4.13 and 4.26 OD/mg protein/min, respectively) and ALT activity (from 1.02 in control to 5.22 and 5.50 OD/mg protein/min, respectively), after 72 h of exposure. In the case of GST, the highest enzyme activity inhibition was occurred by lufenuron at 0.28 and 0.62 ppm, where the inhibition percentage was 38.6 and 45.6, respectively. Results also revealed that, chlorfluazuron caused the highest decrease in total haemocyte count 49.26 and 53.89% at 0.31 and 0.76 ppm, respectively. Changes in the differential haemocyte population during lufenuron and chlorfluazuron treatments have been assessed. The percentage of Prohaemocytes (Pr) markedly decreased after treatment with both insecticides when compared with control but no significant difference was observed between different concentrations of tested insecticides. Moreover, the percentage of Granulocytes (Gr) and Plasmatocytes (Pl) decreased markedly after treatments. In contrast, Oenocytes (Oe) and Spherulocytes (Sph) increased after 72 h of lufenuron and chlorfluazuron treatment. Also, the Corpora Allata (CA) activity has been observed in S. littoralis treated larvae. Both lufenuron and chlorfluazuron decreased the CA surface area after 72 h of treatment compared to control.

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