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Journal of Entomology
Year: 2009  |  Volume: 6  |  Issue: 3  |  Page No.: 167 - 175

New Isolate Media of the Mosquito Pathogenic Fungus Lagenidium giganteum (Oomycetes: Lagenidiales) for Fungal Maintenance and Zoospores Release

M.S. Shathele    

Abstract: Newly formulated Saudi-1 and Saudi-2 media provided highly conspicuous growth and detection of Lagenidium giganteum zoospores on initial plating. The selectivity of Saudi-type media was based on a lecithin (soybean) and antibiotics, streptomycin and ampicillin. The Saudi-1 media supported the vegetative growth of Lagenidium giganteum (under continuous 60 W light source to get conspicuous growth). The Saudi-2 media was more defined and selective than Saudi-1. The liquid proflo PB/3 media was the best growth medium for inducing sporulation. The LC50 values obtained in this study were higher than those reported previously. One possible reason for the discrepancies in LC50 values of L. giganteum zoospores against mosquito larvae might be due to the difference in the handling techniques of treatment concentrations. On the other hand, perhaps a more important factor is the type of water (original medium) used in the previous experiments. The present study utilized sterile volvic water (double distilled), whereas the previous studies used distilled water. In conclusion, new isolate of L. giganteum is reported along with their pathogenicity towards mosquito larvae and their zoosporogenesis pattern. The study showed an excellent potential for future investigations on the use of newly developed isolate Saudi media for mosquito pathogeneic fungus for its maintenance and zoospores release.

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