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Journal of Entomology
Year: 2009  |  Volume: 6  |  Issue: 2  |  Page No.: 117 - 123

Quantitative PCR Detection of Cholinesterase and Carboxylesterase Expression Levels in Acaricide Resistant Rhipicephalus (Boophilus) microplus

R. Cossio-Bayugar, E. Miranda-Miranda, D. Portilla-Salgado and J. Osorio-Miranda    

Abstract: This study was done with the objective of developing a methodology for cholinesterase and carboxylesterase expression measurement in acaricide resistant R. microplus, for that purpose the expression levels for these enzymes were measured by real time PCR quantification of mRNA specific detection, comparing acaricide sensitive with multiple acaricide resistant strains of ticks known as Mora and San Alfonso. Acaricide susceptible ticks were used as standard cholinesterase and carboxylesterase expression level and adjusted as a baseline of 1 Relative Expression Units (REU). A statistical significance was observed in cholinesterase gene expression level as 13.07 ± 3.49 REU for Mora strain and 10.81 ± 2.98 REU for San Alfonso strain compared to the susceptible strain. Also, carboxylestrase expression found statistically significant for Mora and San Alfonso strains (6.9 ± 1.14 and 12.11 ± 1.81 REU, respectively) compared to the susceptible strain. Present results proved that the carboxylestrase and cholinesterase genes expression increased by acaricide pesticide exposure in Mora and San Alfonso, R. microplus, which explained as an overexpression of AchE2 at the singanglion level for OP resistant ticks, as well as increased levels of esterase gene CzEST9 implicated in pyrethroid resistant strains.

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