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Journal of Biological Sciences
Year: 2018  |  Volume: 18  |  Issue: 8  |  Page No.: 415 - 424

Biological Treatment of Carcinogenic Acrylonitrile Using Pseudomonas aeruginosa in Basra City

Sufyan Mohammed Shartooh, Laith Muslih Najeeb and Muthana Mohammed Sirhan    

Abstract: Background and Objective: Biological treatment has become a temptic alternative for expensive ways. However, the low costs and technology of bio-remediation means that it is possible to use for addressing ecological problems in many polluted locations around the world. The objective of this study was to determined the biodegradation ability of Pseudomonas aeruginosa to utilize carcinogenic acrylonitrile, via an environmentally-sound method. Materials and Methods: Comprised isolation of bacteria using suspended soil dilution method, where eight soil samples were collected from polluted sites in the Southern part of Basra city. In return, the best isolation was chosen in its growth on a media containing acrylonitrile as a sole-source of carbon and nitrogen, isolate was identified using biochemical tests that shows it belong to the species of Pseudomonas aeruginosa. However, substantial cultural processes were conducted at this isolate to reveal the preferable period of adaptation. Additionally, series of acrylonitrile (C3H3N) concentrations were prepared within the purity of 96%, a pure culture of bacteria was obtained to determine its efficiency for acrylonitrile degradation and the bacterial-culture was concentrated using enrichment media. Aforementioned concentrations were inoculated with 1.5×108 cell mL–1 of bacterial culture inferenced by McFarland standard (600 nm, OD = 0.5) and were incubated for 7 days at 37°C, then the concentration of acrylonitrile was measured before and after treatment using HPLC and FTIR spectrum, to set the efficiency of biodegradation confirmed by the production of ammonia, through decomposition processes. Results: The under study bacteria have the ability to degrade the carcinogenic material, the best adaptation period was for 7 days as well as the optimum concentration for treatment is 500 ppm, with a degradation efficiency of 92.7%. Conclusion: The extracted results revealed that the isolated bacteria demonstrated eminent quantities of acrylonitrile degradation. Thus, it could be used to bio-remediate contaminated soils in such environments.

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