Anti-angiogenic Effects of Cadmium Chloride on the Process of Neovascularization
Background and Objective: Angiogenesis (neovascularization) is an important process of forming new blood vessels, essential for embryonic development, reproduction, wound repair and growth. Present study reports the plausible angiogenic modulatory effects of the cadmium chloride (CdCl2). Cadmium (Cd) is a toxic substance, which have many inhibitory effects but also medicinally important. There are several toxic materials which in low quantity can be used in therapeutic aspects. Keeping in view, the effects of CdCl2 salt were investigated on the process of angiogenesis in chicken chorioallantoic membrane (CAM) model by assessing gross and histopathological alterations. Materials and Methods: The two groups of the embryonated chicken eggs (10 in each group, 1 control and 1 treated group) were taken and 200 μL of 0.5 M of CdCl2 was directly introduced on to the CAM. The resealed eggs were incubated for 72 h in a humid incubator chamber at 37±1°C. Then the eggs were opened to observe the gross and histopathological alterations for angiogenesis modulation. Results: Gross examination of CAM revealed the reduction in the number of secondary and tertiary blood vessels amongst CdCl2 treated group. Histopathological analysis revealed anti-angiogenic effects of CdCl2 due to the less number of blood vessels and presence of breached mesodermal blood vessels, out of which RBCs were oozing out. Occasional abnormal thickening and accumulation of densely arranged cells at chorionic and allantoic sides was also observed. Conclusion: The anti-angiogenic properties of CdCl2 can be explored in the therapeutics of cancer and tumor related disorders. However, to avoid any kind of side effects, further qualitative, quantitative analysis and critical dose determination is required before going for clinical trials. Present investigations revealed the anti-angiogenic effect of cadmium chloride salt on chicken chorioallantoic membrane.
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