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Journal of Biological Sciences

Year: 2015  |  Volume: 15  |  Issue: 3  |  Page No.: 106 - 115

Microwave Assisted Extraction of Tinospora cordifolia and Optimization through Central Composite Design

Saurabh Satija, Preeti Bansal, Harish Dureja and Munish Garg


Present study was conducted to develop a new optimized Microwave-Assisted Extraction (MAE) method for Tinospora cordifolia in order to improve the efficiency and yield of chief bioactive compounds. Stems of the Tinospora cordifolia were subjected for extraction using MAE technique using three variable factors (extraction time, irradiation power and solvent concentration) optimized through central composite design. Berberine which was used, as marker was estimated in prepared extract by High Performance Thin Layer Chromatography (HPTLC) and compared with extracts prepared by conventional techniques like maceration and soxhlation. The results revealed that MAE of Tinospora cordifolia at 60% irradiation power, 80% ethanol concentration and at 3 min extraction time produced highest extract yield (91.3% better yield than maceration and 25.7% than soxhlation) as well as berberine content (492.8% better than maceration and 59.6% than soxhlation) as compared to extracts prepared with conventional techniques. Efficiency of the MAE method was considerably better than the conventional procedures, especially in terms of shortening extraction time (3 min as compared to 3 h for soxhlation and 7 days fort maceration), reduction of solvent used and energy consumption. The optimized microwave extraction method can provide a valuable extraction alternative of Tinospora cordifolia stem at industrial scale.

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