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Journal of Biological Sciences

Year: 2014  |  Volume: 14  |  Issue: 8  |  Page No.: 521 - 526

Coconut Water from Fresh and Dry Fruits as an Alternative to BAP in the in vitro Culture of Dwarf Cavendish Banana

J.N. Buah and P. Agu-Asare


The ability of coconut water to support the regeneration of banana plantlets in vitro was investigated from March to September 2014 at the Plant Genetic Resource Institute, Bunso, Ghana. The 100 mL L-1 coconut water was compared with 4.5 mg L-1 BAP. The tissues were cultured for 16 weeks at 267°C, 16 h. photoperiod, 3000 Lux light intensity and relative humidity of 60% with data taken on Fresh weight, Dry weight, Shoot number, Shoot height and other parameters. Explants cultured on 4.5 mg L-1 BAP produced 37 shoots/explant while those on 100 mL L-1 coconut water recorded 25 shoots/explant. However, for plant height, plant cultured on medium supplemented with coconut water from fresh green fruits had height of 18 cm but those cultured on medium with BAP had a height of 15 cm at 16 weeks. The differences between the performance of plants on BAP and coconut water were however not significant. Coconut water from the fresh green fruits was observed to be a suitable alternative to BAP in the in vitro culture of banana plants.

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