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Journal of Biological Sciences
Year: 2014  |  Volume: 14  |  Issue: 6  |  Page No.: 414 - 424

Bacterial Community Structures of Planktonic Cells and Biofilm at Saguling Hydro Power using Denaturing Gradient Gel Electrophoresis (DGGE)

Keukeu K. Rosada, Nur F. Afianti, Dea Indriani Astuti, Gede Suantika and Pingkan Aditiawati    

Abstract: Bacterial communities in planktonic cells and biofilm at Saguling Hydro Power were investigated using Denaturing Gradient Gel Electrophoresis (DGGE) technique. Physicochemical characteristic of aqueous medium indicated that the water source was classified as moderately polluted (eutrophic). The study inferred that isolates in biofilm mostly derived from the planktonic cells and the Mann-Withney U tests showed that there was no significantly difference between the bacterial communities of planktonic cells and biofilm. The 16S rRNA sequences revealed that bacteria recovered from the planktonic cells were affiliated with Betaproteobacteria and Bacteroidetes phyla. Meanwhile, the sequences bacteria revealed from biofilm were closed to Betaproteobacteria, Alphaproteobacteria and Gammaproteobacteria groups. Overall, the majority of microbes identified from the two samples were belong to Betaproteobacteria group.

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