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Journal of Biological Sciences
Year: 2013  |  Volume: 13  |  Issue: 2  |  Page No.: 94 - 99

Antifungal Compounds Production in Different Temperatures, pH and on Modified MRS Agar by Lactobacillus Strains

Nora Laref, Bettache Guessas and Mebrouk Kihal    

Abstract: The effect of abiotic and biotic factors on the antifungal activity was evaluated by overlay assay under different temperatures, pH and on modified MRS (de Man Rogosa Sharpe). Lactic Acid Bacteria (LAB) were able to produce antifungal compounds under different temperatures, in 30°C showed a good inhibition because no conidia was observed; in this temperature all isolates when grown at pH 3 and 4 show a stronger antifungal activity. The best inhibition was done when the glucose was replaced with sorbitol, mannitol and trehalose. The same zone of inhibition in the presence and absence of sodium acetate was detected by all isolates. No inhibition was observed on MRS without meat extract and glucose, although the amount of activity produced does vary depending on the carbon source.

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