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Journal of Biological Sciences
Year: 2013  |  Volume: 13  |  Issue: 6  |  Page No.: 528 - 533

Molecular Characterization of SSI Gene in Triticum L. and Aegilops tauschii

Wei Li, An-Jun Liu, Yu-Zhen Sheng, Zi-En Pu, Ya-Xi Liu and Guo-Yue Cheng    

Abstract: In order to knowing the variation of starch synthase I (SSI) in Triticum L. and Aegilops and exploiting new gene resources for the improving of starch quality in common wheat, using PCR cloning, eight sequences of SSI genes were obtained from AS60 (DD, Aegilops tauschii), AS2255 (AABB, Triticum turgidum), SHW-L1 (AABBDD, synthetic hexaploid wheat) and Chuanmai 32 (AABBDD, Triticum aestivum), respectively. These sequences of SSI gene showed the higher conservative characterization between different materials. A total of 10 variable nucleotide bases and two substitutions of amino acid residues (Ser/Asn and Ala/Val) were observed in the cloning sequences. Alignment of all sequences, including SSI gene from T. aestivum, Hordeum vulgare, Brachypodium distachyon, Zea mays and Oryza sativa suggested that the sequences from Ae, tauschii, T. turgidum and synthetic hexaploid wheat, were more similar to SSI gene from common wheat and barley and far distance with sequences from Brachypodium distachyon, Zea mays and Oryza sativa. Using neighbor-joining method, phylogenetic tree including a total of 28 sequences could be clustered into four groups. Most of sequences of SSI from different species were clustered together and SSI gene showed the clear difference with other starch synthases, including Granule-bound Starch Synthase (GBSS), SSII, SSIII and SSIV. These results suggested that there was variation fro SSI gene in Triticum L. and Aegilops. It necessary to developing molecular markers for exploit genetic resources and the improving of wheat starch quality.

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