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Journal of Biological Sciences
Year: 2013  |  Volume: 13  |  Issue: 5  |  Page No.: 422 - 426

Effect of Three Sewage Isolated Bacteriophages on the Multidrug Resistant Pathogenic Bacteria

Nafiseh Sadat Naghavi, Mahdieh Golgoljam and Mojgan Akbari    

Abstract: Bacteriophages have been proposed as specific alternative for chemotherapy against multidrug resistant bacterial infections. The aim of the present study was the isolation and purification of bacteriophages from sewage, which were effective against multidrug resistant bacterial isolates and detection of their morphological characteristics. Sewage and clinical samples were used for the isolation of antibiotic resistant pathogenic bacteria. The bacteriophages were isolated and purified from sewage samples by overlay cultures of the isolated bacteria after centrifugation and filtration procedures. Three isolates of the most multidrug resistant Escherichia coli, Klebsiella pneumonia and Pseudomonas aeruginosa selected for bacteriophage isolation. Most of the isolates were resistant to different classes of antibiotics in the aminoglycosides, cephalosporins, macrolides, tetracyclines and β-lactam antibiotic groups. The isolated bacteriophges showed different plaque morphologies on the bacterial cultures. The three purified bacteriophages had icosahedral or hexagonal heads. Long tails observed in Escherichia coli bacteriophages, with the total length of about 170 nm for the phages particles; short tails observed in Klebsiella pneumonia phages particles, with the approximate total lengths of 150 nm; and short tails with the approximate 35 nm total particles length observed in Pseudomonas aeruginosa bacteriophages.

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