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Journal of Biological Sciences
Year: 2013  |  Volume: 13  |  Issue: 4  |  Page No.: 283 - 287

Isolation, Characterization and Anticoagulant Activity of Sulfated Polysaccharides from Brown Algae Sargasam wightii Greville

R. Saravanan, K. Kumar Ebenezar, S. Rajasekaran and R. Thamaraiselvan    

Abstract: The aim of the present study is to isolate the sulfated polysaccharides from marine macro algae S. wightii. The crude sulfated polysaccharides were fractionated by anionic resin (Amberlite IRA-900) and the separated active fractions were confirmed by agarose gel electrophoresis. The active fractions were pooled, dialyzed and purified by molecular sieve (Sephadex G-100) chromatography. The molecular weight of fractionated as well as purified sulfated polysaccharides was determined through gradient polyacrylamide gel electrophoresis (PAGE) and the disaccharide profile of purified sulfated polysaccharides was also analyzed. The structure of purified sulfated polysaccharides was dogged by 1H-NMR spectrum. The elements such as Carbon, Hydrogen and Nitrogen (CHN) and Activated Partial Thromboplastin Time (APTT) were observed from the purified sulfated polysaccharides. The hexosamine, uronic acid and the CHN content in the purified sulfated polysaccharides were found to be low (except molecular weight) when compared to standard heparan sulfate. The molecular weight of purified sulfated polysaccharides have 6,500 Da and the concentration of disaccharide profile such as 5.52% of uronic acid, 3.47% hexosamine and 5.4% of sulfate. APTT of fractionated and purified sulfated polysaccharides was found to be 24.6 and 31.1 IU mg-1, respectively. These results suggest that the sulfated polysaccharides from S. wightii could be an alternative source of anticoagulant.

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