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Journal of Biological Sciences
Year: 2012  |  Volume: 12  |  Issue: 3  |  Page No.: 154 - 160

Molecular Characterization of Different Cytoplasmic Male Sterile Lines Using Mitochondrial DNA Specific Markers in Rice

P. Khera, R. Priyadarshi, A. Singh, R. Mohan, M.G. Gangashetti, B.N. Singh, C. Kole and V. Shenoy    

Abstract: With the objective of identifying mitochondrial DNA based markers that can distinguish cytoplasmic sources and restorer lines, 7 male sterile and fertile counterparts of Cytoplasmic Male Sterile (CMS) lines and 4 restorers (R) lines were characterized, using 20 universal mitochondrial DNA specific markers. Diverse CMS lines, other than the Wild Abortive (WA) one have been developed at International Rice Research Institute (IRRI), Philippines (IR66707A with cytoplasm of Oryza perennis), Central Rice Research Institute (CRRI), Cuttack (CRMS-32A with “Kalinga” cytoplasm from ‘Dunghansali’) and at Directorate of Rice Research (DRR), Hyderabad (DMS 3A with cytoplasm of Oryza nivara and DMS 4A with cytoplasm of Oryza rufipogon). Traditionally, the differences between the cytoplasmic sources were studied by differential response of the Cytoplasmic Male Sterile (CMS) lines when test crossed with a set of known maintainers and restorers. However, these methods could not distinguish the genetic variation at molecular level, since, CMS trait has been found to be associated to mitochondrial DNA aberration. In the present study, seven potential primers were identified, viz., cox1B, nad4ex1, nad5D, nad1, alp, nad4ex2 and rpS14 which showed polymorphism. The results suggested effective utilization of mitochondrial specific primer pairs in hybrid purity testing and marker aided heterosis breeding in rice.

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