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Journal of Biological Sciences
Year: 2012  |  Volume: 12  |  Issue: 3  |  Page No.: 128 - 137

Low Doses of Gamma Radiation may Impair Testicular Tissue in a Rat Treated CCl4 Model: Role of BM Transplantation

N. Hanafi    

Abstract: Treatment of carbon tetrachloride (CCl4) 1 mL kg-1 in olive oil (1:1) twice a week for 8 weeks to albino male rat caused a significant increase in serum level of alanine transaminase (ALT) and aspartate transaminase (AST). Injury to liver, resulting in loss of its normal physiological/biochemical functions, may adversely affect a secondary organ like testis. In the current study young adult rats were treated by Carbon tetrachloride (CCl4) two times per week and/or continuously whole body γ-irradiated (R) at a dose level 0.5 Gy, two times per week for 8 weeks. The previous groups were treated by bone marrow transplantation (BMT). Plasma estradiol and testosterone concentrations in animals sera were analyzed. Histopathological, apoptosis and necrosis examinations were done in testicular tissues. Either CCl4 or R exposure alone or combined reflect testicular seminiferous tubules atrophy, peritubular fibrosis and apoptotic cells in seminiferous epithelium and Leydig cells. BMT reflect some recurrence of normal structure in testis tissue of CCl4 group. Meanwhile R and CCl4 R groups showed atrophied testicular seminiferous tubules, great interstitial hyperplasia, deposition of collagen fibres around blood vessels and presence of interstitial apoptotic and necrotic tissue cells. CCl4 treatment recorded anon significant change in plasma testosterone and a significant decrease in estradiol concentration. γ-irradiation either alone or combined with CCl4 treatments recorded a significant reduction in testosterone level and significant increase in estradiol concentration. BMT recorded a significant increase in testosterone level and anon significant change in estradiol level following CCl4 or irradiation either alone or combined. In conclusion low doses of γ- radiation impair testicular tissue in a rat treated CCl4 model. BM transplantation recorded increase in this testicular damage.

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