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Journal of Biological Sciences

Year: 2009  |  Volume: 9  |  Issue: 5  |  Page No.: 470 - 475

Antioxidative Properties of Ethyl Acetate Fraction of Globimetula braunii in Normal Albino Rats

J. Okpuzor, H. Ogbunugafor and G.K. Kareem


Globimetula braunii used in Nigerian traditional medicine for managing various diseases was investigated for its antioxidant properties in adult Swiss albino rats. The pulverized plant material was extracted in 80% methanol using Soxhlet apparatus and fractionated with hexane, chloroform, ethyl acetate, butanol and water. The crude and ethyl acetate fractions were evaluated for their effects on activities of superoxide dismutase (SOD), catalase (CAT), gluthathione peroxidase (GSH-Px), lipid peroxidation and triacylglycerol levels in rats treated orally with a daily dose of 200 mg kg-1 for 14 days. Results showed that the ethyl acetate fraction caused a significant increase (p<0.05) in the activities of superoxide dismutase, catalase, glutathione peroxidase and malonyldialdehyde (MDA) levels, while the level of triacylglycerol decreased (p<0.05) compared to control. HPLC analysis showed that the crude and ethyl acetate fractions showed phenolic compounds, apigenin and naringin but only naringin was present in the ethyl acetate fraction. It is likely that endogenous Naringin may be acting in vivo both as an antioxidant and a pro-oxidant in normal albino rats.

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