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Journal of Biological Sciences
Year: 2009  |  Volume: 9  |  Issue: 1  |  Page No.: 37 - 43

Use of Microbiological and Chemical Methods for Assessment of Enhanced Hydrocarbon Bioremediation

Saad A. Alamri    

Abstract: A suite of microbiological tools, complementing the standard chemical analysis, was used for evaluating the effect of steam and surfactant on the rate of hydrocarbon biodegradation. The microbiological monitoring tools were soil microbial counts, soil microbial respiration, and microbial toxicity biosensor. The correlations between these parameters and with the levels of hydrocarbon residues were investigated. The overall assessment showed that bioremediation was an effective method for reducing hydrocarbon concentration. However, the monitoring tools used showed that the steam and surfactant had no significant effect in increasing the rate of hydrocarbon bioremediation or the toxicity reduction comparing with the control. Consequently, alternative techniques for enhancing hydrocarbon bioavailability must be investigated in order to establish a successful bioremediation of heavy hydrocarbons in soil. This study also demonstrated that the combination of different classes of biological and chemical tools would be more effective in monitoring hydrocarbon bioremediation that any single approach.

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