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Journal of Biological Sciences
Year: 2009  |  Volume: 9  |  Issue: 3  |  Page No.: 276 - 279

In vitro Anti-Bacterial Activity of Sweet Basil Fractions Against Helicobacter pylori

Mahboobeh Nakhaei Moghaddam, Mehr-angiz Khajeh Karamoddin and Mohammad Ramezani    

Abstract: In tihis study, the effect of sweet basil fractions on growth of H. pylori was studied by filter paper Disc Diffusion Method (DDM) on egg yolk emulsion agar. Aqueous fraction had no activity against nine tested clinical isolates. Methanol, n-hexane and butanol fractions showed in vitro anti Helicobacter pylori effects. There are no differences (p>0.05) among anti Helicobacter pylori activity of methanol, n-hexane and butanol fractions at concentration of 1 mg, but in lower concentrations, methanol and n-hexane fractions had more antibacterial activity than butanol fraction based on Duncan test. Minimum Inhibitory Concentrations (MICs) of methanol, n-hexane and butanol fractions from leaves of sweet basil were 39.1, 41 and 117.2 μg disc-1, respectively. This study demonstrated that methanol, n-hexane and butanol fractions of sweet basil inhibited the growth of H. pylori strains in vitro.

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