Asian Science Citation Index is committed to provide an authoritative, trusted and significant information by the coverage of the most important and influential journals to meet the needs of the global scientific community.  
ASCI Database
308-Lasani Town,
Sargodha Road,
Faisalabad, Pakistan
Fax: +92-41-8815544
Contact Via Web
Suggest a Journal
Journal of Biological Sciences
Year: 2009  |  Volume: 9  |  Issue: 2  |  Page No.: 128 - 136

Temporal and Spatial Expression Pattern of Four Laminin Alpha Chains in Xenopus laevis

N. Ahmad    

Abstract: The spatial and temporal expression of laminin α1, α3, α4 and α5-chain in the developing Xenopus laevis embryos was characterized using RT-PCR and whole-mount in situ hybridization. RT-PCR analysis shows varying developmental timings of expression of the laminin α-chain mRNA. The α1-chain mRNA was detected from stage 6 embryo and levels increase as the embryo develops to stage 41. α3 and α5-chain mRNA was found to be expressed maternally and also in the developing zygote. There was no maternal expression of the α4-chain and in the zygote, α4-chain mRNA was first detected at stage 14. Whole-mount in situ hybridization on embryos at stage 15, 25 and 33 showed that the α1, α3, α4 and α5-chain transcripts produced similar pattern of localisation. Transcripts were observed in the outer epithelia, somites, developing neural tube and pronephros.

Cited References   |    Fulltext    |   Related Articles   |   Back
  Related Articles

Copyright   |   Desclaimer   |    Privacy Policy   |   Browsers   |   Accessibility