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Journal of Biological Sciences
Year: 2009  |  Volume: 9  |  Issue: 1  |  Page No.: 1 - 11

An in vitro Ovule Culture Technique for Producing Interspecific Hybrid Between Tartary Buckwheat and Common Buckwheat

M. Asaduzzaman, M. Minami, K. Matsushima and K. Nemoto    

Abstract: An interspecific hybrid of the Fagopyrum species F. tataricum cv. CT-1 (2x and 4x) and F. esculentum cv. Botansoba (2x) and GreatRuby (4x) was obtained through in vitro ovule culture. Seven to ten days old ovules were excised and cultured on 1/2 MS and MS media and rescued ovules were subjected to different combinations and concentrations of BA, NAA, IAA and zeatin added individually and in various combinations along with sucrose. Effects of the hormones were used to evaluate their potentialities for direct regenerations, callus induction and callus regeneration through ovule culture in interspecific cross between tartary and common buckwheat. Interspecific hybridization in Fagopyrum used ovule culture two methods development in this study. The result showed that no single medium was adequate to ensure complete development of the fertilized ovules to plantlets, thus necessitating a sequential three step transfer to different media. RAPD-PCR analysis confirmed hybridity of the regenerated plant.

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