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Journal of Biological Sciences
Year: 2008  |  Volume: 8  |  Issue: 3  |  Page No.: 570 - 576

Improvement of Plant Regeneration from Embryogenic Suspension Cell Culture of Japonica Rice

Alina Wagiran, Ismanizan Ismail, Che Radziah Che Mohd Zain and Ruslan Abdullah    

Abstract: Improvement on plant regeneration system from suspension cells culture of different japonica rice cultivar was attempted in the present study. The present study shows that callus induction varied depend on genotype. Nipponbare, Hayahishiki and Fujisaka 5 variety showed 100, 93 and 53% of callus induction respectively on LS medium supplemented with 2,4-D after 4 weeks in culture. Callus proliferation was observed at the scutellum and yellowish in colour. Suspension cell culture was initiated from 4 weeks old callus in N6 liquid medium contained with 3 mg L-1 2,4-D and 1 mg L-1 kinetin for one month with regular subculture. The embryogenic cells were characterized with 1% (w/v) Evans Blue. Primary study shows that shoot regeneration was highest on Nipponbare at 60%, while Hayahishiki and Fujisaka were at 30 and 20%, respectively when culture on MS basal medium contained 3 mg L-1 Kinetin and 0.5 mg L-1 NAA (SRM3). After 6 weeks, the callus covered with green shoot bud and later formed shoot with an average of 3-8 shoot per callus. Increase of shoot regeneration was observed when callus treated with partial desiccation in all variety tested. The regeneration frequency was highest at 76, 70 and 33% when Nipponbare, Hayahishiki and Fujisaka 5, respectively were treated with 48 h desiccation. However, shoot multiplication was low in 0 h desiccation ranging from 10-16%. After 8 weeks in optimal SRM medium, the plantlets was hardened in growth chamber for 2 weeks and later transferred into soil with 100% survival in all variety. The present study shows that rice regeneration system from suspension cells can be improved with modification of plant growth regulators and desiccation.

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