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Journal of Biological Sciences
Year: 2008  |  Volume: 8  |  Issue: 8  |  Page No.: 1333 - 1337

Effect of Vitamin C Supplementation on Oxidative Stress Markers Following Thirty Minutes Moderate Intensity Exercise in Healthy Young Women

M. Karandish, S.T. Rahideh and A.Z. Moghaddam    

Abstract: The aim of this double blind randomized controlled trial was to determine the effect of Vitamin C supplementation on oxidative stress following 30 min moderate intensity exercise. Forty-nine healthy young women randomly assigned into 500 mg day-1 vitamin C supplement (n = 25) or placebo (n = 24) groups for two weeks. Before supplementation and on the day after the intervention period, fasting blood samples were taken. Then all participants ran (1.4-1.7 m sec-1) for 30 min. Third blood samples were taken at the end of exercise. Plasma malondialdehyde (MDA) and vitamin C were measured using HPLC method. Plasma total glutathione was measured with ELISA method. No significant differences were observed in demographic and vitamin C intakes before intervention between groups. Plasma MDA levels decreased and plasma total glutathione increased significantly (p<0.05) in both groups. No significant differences were observed between groups after exercise. There were significant differences in plasma vitamin C concentrations after intervention and exercise between groups. In conclusion, vitamin C supplementation (500 mg day-1) for two weeks does not affect oxidative stress markers following moderate intensity exercise in healthy young women.

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