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Journal of Biological Sciences
Year: 2007  |  Volume: 7  |  Issue: 6  |  Page No.: 918 - 924

Characterization of Infraspecific Electrophoretic Genetic Variation within Vicia sativa Subspecies sativa Population

Adel Ramadan El-Shanshoury    

Abstract: Seed protein banding patterns of twenty accessions of V. sativa subspecies sativa were revealed by SDS-PAGE. These have been used to characterize infraspecific genetic variation between accessions collected from different countries. Protein patterns have indicated the existence of low dissimilarity distance within accessions of the same country and a higher degree of dissimilarity between accessions of different countries.Electrophoretic analyses of five isozyme systems (Est, Cbp, Per, Alp and Got) have been carried out on the twenty accession investigated of this subspecies. Variation in allele frequency and number of loci was recorded revealing the existence of homozygosity and heterozygosity. Accessions of the same origin nearly exhibited the same number of alleles and similar intensity of bands in contrast to those of different origin. The results have been discussed with references to the genetic variability within this subspecies as related to difference in origin.

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