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Journal of Biological Sciences
Year: 2007  |  Volume: 7  |  Issue: 8  |  Page No.: 1436 - 1441

The Influence of Cold Acclimation on Proline, Malondialdehyde (MDA), Total Protein and Pigments Contents in Soybean (Glycine max) Seedlings

Leila Zeinali Yadegari, Reza Heidari and Jirair Carapetian    

Abstract: In this study plant`s response to cold acclimation and nonacclimation was investigated in soybean (Glycine max). Seedlings were exposed to 15°C (cold-acclimated) or 25°C (nonacclimated) for 24 h, under 250 μmol m-2 sec-1 Photosyntetically Active Radiation (PAR). Then, all plants were exposed to chilling temperature at 4°C for 24 h and allowed to recover at 25°C for 24 h. Physiological responses to chilling, including, MDA, proline, chlorophyll a and b and carotenoids and total protein contents were measured in soybean to identify mechanisms of chilling tolerance. Relative water content showed that cold-acclimated plants were less affected by chilling compared to nonacclimated plants. Cold-acclimated plants also recovered faster from chilling injury than nonacclimated plants.

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