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Journal of Biological Sciences
Year: 2007  |  Volume: 7  |  Issue: 7  |  Page No.: 1266 - 1269

Biosurfactant Production by the Strain Isolated from Contaminated Soil

Nasrin Samadi, Mohammad Reza Fazeli, Neda Abadian, Abbas Akhavan, Abbas Tahzibi and Hossein Jamalifar    

Abstract: A bacterial strain was isolated from vegetable oil contaminated soil, identified and named as Brevibacterium sp. strain S-34. Hemolysis of erythrocytes and emulsification of the oil in culture media indicated surface active agents production by the strain. Thin layer chromatography studies revealed that the secreted biosurfactants were glycolipids. The potential production of biosurfactants is assessed using different carbon sources (glucose, glycerol, molasses, gasoline, Canola oil and waste oil). Optical density, pH and surface tension were employed to determine the biosurfactant activity of strain S-34 in mineral salts medium with each carbon source. The microorganism was able to grow well on glycerol (50 g L-1) and reducing the surface tension of medium from 69 mN m-1 to 30 mN m-1 by production of 2.4 g L-1 total biosurfactant after 72 h.

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