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Journal of Biological Sciences
Year: 2007  |  Volume: 7  |  Issue: 7  |  Page No.: 1147 - 1154

Serological Differentiation Indices and Phylogenetic Analysis ofRice yellow mottle virus Isolates in Cote d`Ivoire

Y. Sere, A. Onasanya, K. Akator, A. Afolabi and M.E. Abo    

Abstract: Serological diversity of 178 RYMV isolates was determined by phylogenetic analysis of Serological Differentiation Indices (SDI) data generated from antigen coated-plate enzyme-linked immunosorbent assay (ACP-ELISA) using 26 RYMV Polyclonal antisera. These RYMV isolates were obtained from northern, southern, eastern and western Cote d`Ivoire. All the RYMV isolates was classified into three main serogroups (Sg1, Sg2 and Sg3) and six subgroups (Sg1a, Sg1b, Sg2a, Sg2b, Sg3a and Sg3a). This indicates the existence and levels of serodiversity among RYMV isolates in Cote d`Ivoire. These results provide evidence of a possible relationship between serological property, host plant and ecological origin of RYMV isolates. Phylogenetic classification of each RYMV isolate defined by SDI data in ACP-ELISA is potentially useful in epidemiological studies to assess isolate identity and interaction as well as to assist breeding programs aiming at the development of cultivars with durable resistant to RYMV in Cote d`Ivoire.

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