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Journal of Biological Sciences
Year: 2006  |  Volume: 6  |  Issue: 4  |  Page No.: 687 - 694

Optimization of Culture Conditions for Production of Polyamide Biopolymer (Polyglutamate) by Bacillus sp. Strain-R

Mahmoud M. Berekaa, Yasser R. Abdel-Fattah, Samia M. El-Sayed, Aliaa M. EL Borai and Samy A. El Aassar    

Abstract: An optimization study of biopolymer production by locally isolated Bacillus sp. strain-R was carried out, where analysis indicated that it is a polyamide homopolymer consists mainly of glutamate. Preliminary experiment to address the most suitable carbon and nitrogen sources revealed that a production of 14.25 g L-1 of the polyamide (polyglutamate) was achieved in the presence of glucose and ammonium sulfate. To evaluate the effect of different culture conditions on the production of PGA, Plackett-Burman factorial design was carried out. Ten variables were examined for their significance on PGA production. Among those variables K2HPO4, KH2PO4, (NH4)2SO4 and glucose were found to be the most significant variables that encourage PGA production. The pre-optimized medium showed approximately three folds increase in PGA production.

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