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Journal of Biological Sciences
Year: 2006  |  Volume: 6  |  Issue: 4  |  Page No.: 680 - 686

Report on Some of the Physical Properties of Bioactive Compounds Responsible for the Channa striatus Fillet Extract Antinociceptive Activity

Z.A. Zakaria, A.M. Mat Jais, M.N. Somchit, M.R. Sulaiman and C.A. Fatimah    

Abstract: The present study was carried out to elucidate some of the basic physical properties of the bioactive compounds responsible for Channa striatus antinociception. The aqueous extract of C. striatus was obtained after soaking the fish fresh fillet in the ratio of 1:2 (w/v) in chloroform: methanol (2:1; v:v) overnight. The extract, in the concentrations of 10, 50 and 100%, was subjected to the centrifugation-filtration process using the Millipore Ultrafree-CL low binding cellulose filters with different pore size (5,000, 10,000 and 30,000 dalton) prior to the abdominal constriction test in mice, respectively. Subsequently, the 100% concentration extract was also subjected to the analytical high performance liquid chromatography (using a Gilson 715 HPLC equipped with two slave 306 pumps and analytical reverse-phase Vydac C-18 column (10 μm, 0.46 x 25 cm) set at 40°C, with the flow rate of 0.5 mL min-1 absorbance at the wavelength of 220 nm was monitored by a wavelength UV detector; the eluant A was 100% methanol and eluant B was 80% methanol in dH2O) to establish its chromatogram profiles and the first fraction obtained was collected and subjected to the same antinocceptive assay. The non-filtered extract was found to exhibit a concentration-dependent antinociception (p<0.05). Except for the 30,000 dalton extract, the 5,000 and 10,000 dalton extracts antinociception were significantly different (p<0.05) from the non-filtered extract. Furthermore, the chromatogram profiles of C. striatus aqueous extract revealed the presence of four major fractions while the first fraction, prepared in the concentrations of 0.005, 0.05, 0.5 and 5 mg kg-1, was found to exhibit significantly (p<0.05) concentration-dependent antinociception. Based on the present data obtained, it is plausible to suggest that at least four types of bioactive compounds with molecular weight ranging from lower than 5,000 dalton as well as between 10,000 to 30,000 dalton presences in the aqueous extract of C. striatus.

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