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Journal of Biological Sciences
Year: 2006  |  Volume: 6  |  Issue: 2  |  Page No.: 286 - 293

Comparison of House Fly Forewing and Artificial Media for Examining Environmental Effects on Conidial Germination of Entomopathogenic Fungi

Mohammed El Damir    

Abstract: The objectives of this study were to test the use of a forewing of the house fly (Musca domestica L.), ¼ strength sabauroud dextrose agar (SDAY/4) and dry agar-agar as substrates to assess the effect of relative humidity (RH) (91, 95, 98 and 100 ± 1%) and temperature (15, 20 and 30 ± 1 °C) on spore germination of Beauveria bassiana 5672 and Metarhizium anisopliae 1080 isolates at 24, 48 and 72 h post exposure. In all treatments tested, similar influences of temperature and %RH were observed when conidial germination of 5672 and 1080 was tested on SDAY/4 and fly forewing, whereas little or no germination occurred with agar-agar as a substrate. Conidial germination occurred at temperatures of 15-30 °C and >=95% RH and temperatures of 20-30 °C and >95% RH for B. bassiana 5672 and M. anisoplia 1080, respectively. At 100% RH, 30 °C and 72 h exposure period, the highest germination rates of 1080 were 99 and 96% on SDAY/4 and fly forewing, respectively. We concluded that the SDAY/4 medium triggered the conidial germination of these fungi similar to the house fly forewing cuticle. This suggests that SDAY/4 medium is useful as a substitute substrate of cuticle when examining the influence of temperature and %RH on conidial germination. The SDAY/4 medium is also nutritive and supplies a substrate for growth and sporulation. This technique provides a tool for predicting the epidemiology of these fungi in the field for an IPM program of Sunn Pest.

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