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Journal of Biological Sciences
Year: 2006  |  Volume: 6  |  Issue: 2  |  Page No.: 269 - 274

Effect of Growing Media and Water Volume on Conidial Production of Beauveria bassiana and Metarhizium anisopliae

Mohammed El Damir    

Abstract: The objective of this study was to evaluate conidial production of two isolates of Beauveria bassiana (Balsamo) (5672 and CA 44) and one of Metarhizium anisopliae (Metschnikoff) (500B) using corn, wheat and millet and three water volumes (substrate: water) (1:0.5), (1:1) and (1:1.5). We found that M. anisopliae tended to produce more conidia overall than the B. bassiana isolates. For the B. bassiana isolates, conidial production on wheat was higher for the 1:1 and 1:1.5 ratio than the 1:0.5. No significant differences were found in conidial production for 5672 on corn and millet at the volume regimes tested; whereas, for CA-44 on corn and millet, conidial production tended to decrease with increasing water volume. In contrast, conidial production of M. anisopliae increased up to 8.6 and 2.9 times between the lowest and highest water volume ratio on corn and millet, respectively. Wheat tended to produce more conidia depending on the water volume used, although only slight increases were observed for 5672. We found that conidial production was positively influenced by increased water ratio, particularly for M. anisopliae (500B). Depending on the combination of isolate, growing media and water volume, differences up to 240 times were observed. Such difference can have dramatic effect on the economical production of entomopathogenic fungi for field application. Present results provide useful information for developing a simple mass production tactic for targeting Sunn Pest in their overwintering sites.

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