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Journal of Biological Sciences
Year: 2006  |  Volume: 6  |  Issue: 1  |  Page No.: 193 - 197

Process Optimization for ß-D-Galactosidase Production Using Yeast Culture

R. Panesar, P.S. Panesar, R.S. Singh, J.F. Kennedy and M.B. Bera    

Abstract: The use of Kluyveromyces marxianus NCIM 3465 for the production of ß-D-galactosidase is reported. Experimentation was carried out to optimize the process parameters for maximum enzyme production by varying the parameters such as pH of the medium, temperature, inoculum size, age of inoculum, agitation and incubation time. The maximum enzyme activity was observed with pH 5.0, temperature 30°C, inoculum size 6% (v/v) having 20 h age, under shaking conditions (100 rpm) after 24 h of incubation.

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