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Journal of Biological Sciences
Year: 2005  |  Volume: 5  |  Issue: 4  |  Page No.: 486 - 489

Callus Induction and Indirect Regeneration in Peltophorum pterocarpum (DC.) Backer ex K. Heyne

M. Salah Uddin, K. Nasirujjaman, M.M. Rahman and M.A. Reza    

Abstract: The present study was conducted to induce callus from different in vitro grown seedling explants viz., cotyledon, nodal segment and leaf segment and to develop shoot buds from callus. At first, all the explants were cultured on MS medium supplement with different concentrations and combinations of BAP, KIN, NAA and 2,4-D. Among all the explants, cotyledon showed best performance for callus induction. For this explant, the highest callusing (93.33%) and the highest fresh weight of callus (1.38 g) was observed in MS+2.0 mg L 1 BAP+0.5 mgL 1NAA media composition. Among all the concentrations and combinations, higher concentration of cytokinin (BAP) with lower concentration of auxin (NAA) was proved to be the best for callus induction. Calli, which were obtained from cotyledon and leaf segment in MS+2.0 mg L 1 BAP+0.5 mg L 1 NAA were subcultured in MS media having different concentrations and combinations of BAP and KIN with NAA. It was found that MS medium supplemented with KIN-NAA combination was the best for development of shoot buds from calli.

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