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Journal of Biological Sciences

Year: 2005  |  Volume: 5  |  Issue: 3  |  Page No.: 300 - 304

In vitro Rapid Propagation of Black Thorn (Kaempferia galanga L.): A Rare Medicinal and Aromatic Plant of Bangladesh

M.M. Rahman, M.N. Amin, T. Ahamed, S. Ahmad, A. Habib, R. Ahmed, M.B. Ahmed and M.R. Ali

Abstract

An efficient protocol has been established for rapid production of plantlets using rhizome tip and lateral bud explants of the field grown plant. The explants were cultured on MS medium with auxins (NAA, IBA and IAA) and cytokinins (BA and Kn). Cent percent of the explants produced two or three shoot buds in each culture when they were cultured on MS medium containing 1.0 mg L -1 BA+0.1 mg L -1 NAA within three weeks of culture. The number of shoots per culture increased gradually when the primary cultures were subcultured in two weeks intervals. Highest number of 20.50±1.80 shoots proliferated in each culture when the explants of initially sprouted shoots were subcultured at three times on the same medium. Microshoots were isolated from the in vitro proliferated cluster of shoots produced roots in 100% cases on modified (MMS2) medium supplemented with 0.2 mg L -1 of IBA. Maximum number of 12.4±1.23 roots per microshoot were recorded on the medium containing 0.2 mg L -1 IBA. The regenerated plantlets were acclimatized and established on the soil with eighty five percent success.

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