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Journal of Biological Sciences
Year: 2004  |  Volume: 4  |  Issue: 6  |  Page No.: 731 - 734

Homology of Seed Coat Color Specific Marker of B. juncea with Brown Seeded Cultivar of B. rapa

Rahman Md. Mukhlesur and Yutaka Hirata    

Abstract: Polymerase Chain  Reaction (PCR) was carried out with seed coat color specific SCAR marker of B. juncea. The yellow-seeded cultivars were collected from Japan and Denmark, where the brown-seeded cultivars were collected from Bangladesh and Japan. PCR products were visualized in 1% agarose gel, where the yellow-seeded cultivars produced a strong band at 0.5 kb and weak band at 1.2 kb. In addition of these two specific bands, the Japanese yellow-seeded cultivars expressed two more week bands at 1.1 and 1.0 kb. The brown-seeded cultivars generated a single strong band at 1.1 kb. In segregating population of a cross of yellow-seeded and brown seeded cultivars, the yellow-seed coat color marker segregated at a ratio 15 (brown): 1 (yellow), indicating the digenic inheritance pattern of the traits. PCR was also carried out with the same primers to the yellow-seeded and brown-seeded cultivars of B. rapa. It was interesting to note that the yellow-seeded cultivar of B. rapa did not show any band, where the brown-seeded cultivar of B. rapa showed bands at 1.1 kb and 0.5 kb. The result indicates that the seed coat color specific primer of B. juncea has also ability to differentiate the yellow-seeded and brown-seeded cultivars of B. rapa. PCR-Southern hybridization was carried out with 0.5 and 1.1 kb fragments as prove to check the homology of the other amplified fragments. The prove DNA fragments (0.5 or 1.1 kb) hybridized all other DNA fragments at 1.2, 1.1, 1.0 and 0.5 kb of B. juncea and brown-seeded B. rapa. The result indicating that all the mentioned fragments bearing a common homology region of DNA of yellow-seeded B. juncea and brown-seeded cultivars of B. rapa.

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