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Journal of Biological Sciences

Year: 2004  |  Volume: 4  |  Issue: 3  |  Page No.: 298 - 303

In vitro Rooting Performance of Native-olive (Elaeocarpus robustus Roxb.) under Different Auxins and High Temperature Treatments

M.M. Rahman, M.N. Amin, S. Ahmad and R. Ahmed

Abstract

Micropropagation of native-olive (Elaeocarpus robustus Roxb.) has been proved difficult due very slow rooting of the shoot cuttings. The objective of the present investigation was to develop a technique for in vitro fast rooting of the aseptically grown shoots of native-olive. Rooting experiment was conducted on 1/2MS medium with various concentrations and combinations of NAA, IBA and IAA along with or without incubation at higher temperature (30 ° C) in dark condition for initial one week. Root forming performance of IBA was proved to be the best among the three auxins tested. Highest frequency of rooting with maximum number of effective roots was observed on the hormone-free medium in normal growth room condition after treating the microcutting with 0.2 mg L-1 IBA and incubated under 30 ± C in dark for initial one week. Early emergence and fast growth of roots without any malformation were also observed at the same treatment. At this culture condition 85% of the microcuttings produced 5.80 ± 0.15 roots per cutting where average length of roots per cutting was 4.65 ± 0.18 cm. The plantlets originated through the above treatment established themselves under ex vitro condition much quicker than those originated through other treatments.

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