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Journal of Biological Sciences

Year: 2004  |  Volume: 4  |  Issue: 2  |  Page No.: 219 - 223

Plant Regeneration of Withania somnifera (L.) Dunal (Ashwagandha) from Nodal Segments Derived Callus an Endangered Medicinal Plant in Bangladesh

N.A. Siddique, M.A. Bari, Sharmin Shahnewaz, M.H. Rahman, M.R. Hasan, M.S.I. Khan and M.S. Islam

Abstract

Callus cultures were initiated from nodal segments on Murashige and Skoog[19] medium supplemented with 2,4-D, BAP and Kn. The highest frequency (85%) of organogenic callus induction was observed in MS medium containing 1 mg L ha-1 BAP and 2 mg L ha-1 Kn. Development of adventitious shoots occurred when the calli were subcultured in MS medium supplemented in the BAP and Kn. Shoots differentiated best (80%) from node derived callus on MS medium containing 1 mg L ha-1 BAP and 2.5 mg L ha-1 Kn. Regenerated shoots rooted best on MS medium containing IBA and Kn (1 mg L ha-1 ). Plantlets were transferred to pots containing sand and soil mixture, acclimatized in a culture room and finally rooted plants were transferred to soil.

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