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Journal of Biological Sciences
Year: 2004  |  Volume: 4  |  Issue: 2  |  Page No.: 189 - 191

In vitro Regeneration of Garlic Through Callus Culture

N. Khan, M.S. Alam and U.K. Nath    

Abstract: Garlic (Allium sativum) root tips were induced to regenerate shoots through callus culture and somatic embryogenesis to obtain plantlets. The experiment was designed to investigate the regeneration potentiality of two garlic verities and also to develop an efficient protocol for regeneration of garlic via callus culture. Higher percentage of callus was initiated from the combination of 5 mg l ha-1 Kinetin and 1.5 mg l ha-1 2,4-D. Embryogenic callus produced higher number of shoots in MS medium supplemented with 10 BAP. Rooting of individual shoots was induced after transfer to medium without growth regulator. The plantlets were established in the soil after acclimization. Cultivar differences in regeneration from root tips were observed.

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