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Journal of Biological Sciences

Year: 2004  |  Volume: 4  |  Issue: 2  |  Page No.: 146 - 150

Effect of Chayotte Extract on the Biochemical Determinations and on Molecular and Cellular Levels

M.L. Gomes, E.A.C. Lima, R.L. Jales, M. Castro Faria, M. Bernardo-Filho and G. Dire

Abstract

The use of natural products occurs around the world. The labeling of blood constituents with technetium-99m (99m Tc) has been influenced by natural extracts. We evaluated the influence of a chayotte(Sechium edule) extract on the labeling of blood elements with 99m Tc, in the structural conformation of DNA, in the biochemistry of blood and in the measurement of. blood pressure. The animals were treated with chayotte during 15 days and samples of blood were withdrawn. The samples were incubated with stannous chloride and with 99mTc. Plasma(P) and blood cells (BC) were isolated, also precipitated with trichloroacetic acid and soluble(SF) and insoluble fractions(IF) separated. There was a decrease in the radioactivity in IF-P(from 83.96 ± 4.28 to 53.26 ± 6.69). Samples of blood from the treated group were carried out with specific biochemistry kits and the blood biochemistry analysis compounds was done. It was analyzed the level of uric acid, albumin, cholesterol, creatinine, glucose, high density lipoprotein (HDL), globulin and trigliceridics. The gauging of the blood pressure of the animals was taken. Our results showed a reduction on the level of glucose (from 118.40 ± 10.69 to 97.20 ± 4.32) and globulin (3.52 ± 0.13 to 3.08 ± 0.19) as well as in the diastolic pressure (from 123.80 ± 9.12 to 84.40 ± 3.85). It was observed that the referred extract has induced lesions on the DNA molecule. The effect of chayotte extract probably, could be explained by the metabolization of the chayotte that could be capable to induce the generation of active metabolites with oxidant properties.

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