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Journal of Biological Sciences
Year: 2003  |  Volume: 3  |  Issue: 10  |  Page No.: 915 - 920

Effect of Auxin, Sucrose and pH Level on in vitro Rooting of Callus Induced Micro Shoots of Sugarcane (Saccharum officinarum)

R. Baksha, R. Alam, M.Z. Karim, Sk. A. Mannan, B.P. Podder and A.B.M.M. Rahman    

Abstract: Microshoots were cultured on MS medium supplemented with different combinations of auxin, sucrose and pH level for in vitro rooting of callus induced sugarcane (Saccharum officinarum) variety namely Isd 31. Among the types and concentrations of auxin used the best rooting in all the microshoots was obtained with half strength (major salts) MS basal medium supplemented with NAA at 5.0 mg l‾1, sucrose at 50 g l‾1 and pH level 5.7 were the optimum conditions for rooting when microshoots were incubated 25+ 2°C under 16 h photo period regime. The regenerated plantlets were successfully transferred to soil and the percentage of survivability under ex vitro conditions was 75.

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