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Journal of Biological Sciences

Year: 2003  |  Volume: 3  |  Issue: 8  |  Page No.: 751 - 759

In vitro Plant Regeneration from Cotyledon-derived Callus of Three Varieties Pummelo (Citrus grandis [L.] Osb.)

F. Begum, M.N. Amin, S. Islam, M.A.K. Azad and M.M. Rehman

Abstract

Cotyledon explants from in vitro grown seedlings were cultured on half-strength of MS medium with different growth regulators for in vitro indirect regeneration of shoots. Optimum callus formation, when cotyledon explants were cultured on MS containing 1.0 mg l-1 BAP with 5.0 mg l-1 NAA. After that the calli were used for shoot regeneration by transferring them half-strength MS medium supplemented with only cytokinin. Maximum percentage of shoot regeneration was obtained on half strength MS medium in the presence of 1.0 mg l-1 BAP from callus in three varieties [Var.-1 (pink colour), Var.-2 (white colour) and Var.-3 (red colour)] of pummelo. For rooting, shoot cuttings were cultured on half strength MS salts with 0.1-1.0 mg l-1 NAA, IBA or IAA. The best and healthy rooting was observed on 0.1 mg l-1 NAA. The plantlets of three varieties were successfully established on soil. About 95% of plantlets survived under ex vitro condition.

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