In vitro Rapid Regeneration of Plantlets from Leaf Explant of Native-olive
(Elaeocarpus robustus Roxb.)
Leaf segments from field
grown mature plants of Elaeocarpus
robustus Roxb. were used to initiate cultures for inducing callus
and subsequent differentiation of shoot buds. Callus production was
obtained within seven weeks and adventitious shoot formation was observed
after five weeks of incubation on ½ MS medium supplemented
with 0.5 mg l-1 BA + 0.1 mg l-1 NAA + 15% CW.
Complete plantlets were obtained upon transfer of shoot cuttings on
½ MS with 0.1-2.0 mg l-1 IBA, NAA, IAA or without
any auxin. The best and healthy rooting was observed on ½
MS medium supplemented 0.2 mg l-1 IBA. In vitro regenerated plantlets were
transferred to potting soil and successfully established under natural
condition with about 60% survival.
Cited References Fulltext