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Journal of Biological Sciences

Year: 2003  |  Volume: 3  |  Issue: 2  |  Page No.: 172 - 182

Rapid Clonal Propagation of a Medicinal Plant - Adhatoda Vasica Nees. Using Tissue Culture Technique

M.A.K. Azad, M.N. Amin and F. Begum

Abstract

The proliferating axillary shoot cultures were established on MS medium supplemented with different concentrations of cytokinins and auxins, using nodal explants from the field grown mature plant of A. vasica. In vitro response of the explants to multiple shoot regeneration varied greatly with the position of the explanting branch on the donor plant. Highest frequencies of shoot formation and maximum number of shoots per explant were obtained on MS medium supplemented with 0.5 mg/l BA. The elongated shoots were rooted successfully on half strength of MS medium with 0.1-0.2 mg/l IBA or without any auxin. The complete plantlets thus regenerated in vitro were successfully transferred to the field.

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