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Journal of Biological Sciences

Year: 2003  |  Volume: 3  |  Issue: 12  |  Page No.: 1107 - 1113

Rapid Clonal Propagation of A Native-olive @ (Elaeocarpus robustus Roxb.) Using Tissue Culture Technique

M.M. Rahman, M.N. Amin and S. Ahmad

Abstract

Shoot tip and nodal segments from the field grown mature plants of native-olive (Elaeocarpus robustus Roxb.) were used as explants and were cultured on half strength of MS medium supplemented with different types of growth regulators either alone or in combinations. Among the growth regulators and supplements (BA, Kn, NAA and coconut water) that were used in the proliferation medium the best result was observed on 2 MS medium supplemented with 0.5 mgl G 1 BA + 0.5 mgl G 1 Kn + 0.1 mgl G 1 NAA + 15% CW, which promoted multiple shoot bud formation and sufficient shoot elongation. The well-developed shoots were excised to 3-4 cm cuttings and implanted individually on root induction medium. Highest percentage of micro cuttings showed root formation when they were cultured on 2 MS medium containing 0.2 mgl G 1 IBA and incubated under 30° C in dark for initial one week. The in vitro regenerated plants were successfully established in pot holes containing coco-peat on plastic tray and maintained under polythene tents. Survival of the plantlets under ex vitro condition was 60%.

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