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Journal of Biological Sciences
Year: 2003  |  Volume: 3  |  Issue: 12  |  Page No.: 1101 - 1106

Callus Induction and Regeneration from Nodal Segment of Potato Cultivar Diamant

N. Khatun, M.A. Bari, R. Islam, S. Huda, N.A.Siddique, M.H. Rahman and M.U. Mollah    

Abstract: The nodal segments of Diamant cultivar of potato from in vitro grown plantlets were cultured for callus induction and regeneration on MS semisolid medium supplemented with different concentrations of 2,4-D, NAA, BAP alone and NAA with BAP. Highest 90.00% of callus formation was observed in MS+2.5 mgl G 12,4-D. The second highest 83.33% callus induction was recorded in MS+5.0 mgl G 1 BAP. Maximum percentages (70.00%) of calli-induced shoots were observed in MS medium fortified with 5.0 mgl G 1 BAP+0.1 mgl G 1IBA. The regenerated shoots were rooted on MS and 2 MS medium containing different concentrations of IBA and maximum rooting response was achieved in 2 MS +1.0 mgl G 1IBA. Regenerated plants were successfully established in soil after acclimatization.

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