Abstract: The nodal segments of Diamant cultivar of potato from in vitro grown plantlets were cultured for callus induction and regeneration on MS semisolid medium supplemented with different concentrations of 2,4-D, NAA, BAP alone and NAA with BAP. Highest 90.00% of callus formation was observed in MS+2.5 mgl G ¹2,4-D. The second highest 83.33% callus induction was recorded in MS+5.0 mgl G ¹ BAP. Maximum percentages (70.00%) of calli-induced shoots were observed in MS medium fortified with 5.0 mgl G ¹ BAP+0.1 mgl G ¹IBA. The regenerated shoots were rooted on MS and 2 MS medium containing different concentrations of IBA and maximum rooting response was achieved in 2 MS +1.0 mgl G ¹IBA. Regenerated plants were successfully established in soil after acclimatization.