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Journal of Biological Sciences

Year: 2003  |  Volume: 3  |  Issue: 11  |  Page No.: 1058 - 1062

Shoot Proliferation of Dendrobium Orchid with BAP and NAA

S.K. Talukder, K.M. Nasiruddin, S. Yasmin, L. Hassan and R. Begum

Abstract

The experiment was carried out in the Biotechnology Laboratories Departments of Biotechnology and Genetics & Plant Breeding, Bangladesh Agricultural University, Mymensingh during July-November 2002, to investigate the effect of BAP and NAA on orchid shoot regeneration. In this experiment shoot proliferation, root formation, leaf number, increment of shoot length and days required for shoot proliferation of orchid cultured on MS media containing different concentrations of BAP (0, 1, 2.5 and 5 mg l‾1) and NAA (0, 0.1, 0.5 and 1 mg l‾1) were observed. The results revealed that the use of different concentrations of BAP and NAA had a significant effect. Among the concentrations, the best shoot proliferation (1.90/explant), root formation (1.93/explant) leaf number (4.25/plantlet), increment of shoot length (0.472 cm) and the least time requirement for regeneration for regeneration (8.8 days) was obtained from 2.5 mg l‾1 BAP + 0.5 mg l‾1 NAA. At the same time, the lowest shoot proliferation (0.05/explant) was found in control and 0.1 mg l‾1 NAA, Leaf number (0.4/plantelt) from 5 mg l‾1 BAP, increment of shoot length (0.252 cm) from control and highest days requirement (23.8 days) from 5 mg l‾1 BAP +0.1 mg l‾1 NAA was found.

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