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Journal of Biological Sciences
Year: 2002  |  Volume: 2  |  Issue: 2  |  Page No.: 70 - 72

Enhancement in Citrate Production by Alcoholic Limitation

Sikander Ali, Hamad Ashraf and Ikram-ul-Haq    

Abstract: Citric acid fermentation by Aspergillus niger GCB-47 was carried out in a 15L stainless stirred fermentor. Among the alcohols tested as stimulating agents, 1.0% methanol was found to give maximum amount of anhydrous citric acid (76.02 g/l), 24 hours after inoculation. This yield of citric acid is 1.76 fold higher than the control. The sugar consumed and %age of citric acid on the basis of sugar used were 108 g/l and 70.39 %, respectively. The mycelia were small round pellets having dry cell mass 14.5 g/l. Addition of ethanol, however, did not found to enhance citric acid production, significantly.

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