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Journal of Biological Sciences

Year: 2001  |  Volume: 1  |  Issue: 6  |  Page No.: 507 - 510

Effect of Physiochemical Treatments on Brevibacterium flavum for Production of Lysine

Shakeela Naz, Tahira Iqbal, Munir Ahmad Sheikh, Muhammad Shahid and Abdul Ghaffar


Brevibacterium flavum maintained at 7.0 pH level and at 37 °C temperature, gives maximum production of lysine. When the organism was given UV shocks for 30 minutes, gave the lysine in maximum quality. Water substrate ratio, addition of molasses, addition of corn steep liquor were optimized. At 40% water substrate ratio, addition of 4% corn steep liquor and 4% molasses bacteria was most efficient in the production of lysine. The biomass thus produced contained 32.8% crude protein, 20.87% true protein, 34% ash and 1.1% fat contents on the basis of dry matter. There was no increase or decrease in ether extract and crude fiber. The biomass produced was analyzed by three-enzyme method. Its digestibility was 85.69% in single cell protein biomass. Amino acid profile showed that lysine production was 21.48mg/g on the basis of protein.

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