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Journal of Biological Sciences
Year: 2001  |  Volume: 1  |  Issue: 11  |  Page No.: 1078 - 1080

Screening of Exotic Pepper Lines Against Local Isolate of Chili Veinal Mottle Potyviru

Shah, H. and S. Khalid    

Abstract: To identify source of resistance against the most important chili virus viz. chili veinal mottle virus (CVMV), eleven exotic chili lines (CV-1, CV-2, CV-3, CV-5, CV-6, CV-7, CV-8, CV-10, CV-11, CV-12, & CV-21) were screened in controlled conditions. The isolated virus was collected from Sindh province and maintained on Nicotiana tabacum cv. Samsun by successive mechanical inoculations confirmed by double antibody sandwich Enzyme-Linked Immunosorbent Assay (DAS-ELISA) at each step. The seedlings were raised in sterilized soil mixture of peat, clay and sand. Plants were mechanically inoculated (1:4 w/v) at 3-4 leaf stage and the new shoots were tested through DAS-ELISA two weeks post inoculation. The ELISA negative plants were decapitated and the new sprouting were re-inoculated and ELISA was performed two weeks after inoculation. Six lines (CV-1, CV-2, CV-3, CV-7, CV-11 & CV-12) showed no symptoms and were ELISA negative, while four lines (CV-5, CV-6, CV-10, & CV-21) displayed variable reaction resulting to 5, 13, 16 and 22% infection respectively while the susceptible check (CV-8) showed severe mottling with stunted growth (100 % infection) that gave high virus titer (>2). So, asymptomatic and ELISA negative lines showed

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